Cell-Intrinsic Role for NF-kappa B-Inducing Kinase in Peripheral Maintenance but Not Thymic Development of Foxp3+ Regulatory T Cells in Mice
Figure 2
Normal Foxp3+ Tregs and CD25+Foxp3- Treg precursors in thymus of WT recipients of NIK KO BM.
Single-cell suspensions of nucleated thymocytes from NIK KO or WT BM chimeric mice were stained with the indicated fluorescently conjugated antibodies and analyzed by flow cytometry. A, Top plots are gated on donor CD45.1- lymphocytes (BM derived, NIK KO or WT littermate); bottom plots are gated on CD45.1- SP4. Numbers indicate percent of gated cells. B, Quantitation of cell percentages among CD45.1- lymphocytes or SP4. C-E, Quantitation of absolute number of SP4 Foxp3+ CD45.1- thymocytes, total CD45.1- thymocytes, and SP4 CD45.1- thymocytes per mouse, respectively. F, Quantitation of Treg precursors as a percent of CD45.1- SP4. G, Overlay of CD25 expression gated on CD45.1- SP4 Foxp3+; bold line, NIK KO; narrow line, WT. Data in bar graphs represent mean ± SEM of n=3-4 mice per group in one representative experiment of two.