Regulation of Dendritic Filopodial Interactions by ZO-1 and Implications for Dendrite Morphogenesis
Figure 5
Co-localization of the components of the nectin and cadherin systems with ZO-1 in cultured hippocampal neurons.
Cultured hippocampal neurons on 7 DIV were triple-stained for F-actin, ZO-1 and one of nectin-1, nectin-3, afadin, N-cadherin, β-catenin or αN-catenin. (A1) F-actin, ZO-1 and nectin-1; (A2) F-actin, ZO-1 and nectin-3; (A3) F-actin, ZO-1 and afadin; (B1) F-actin, ZO-1 and N-cadherin; (B2) F-actin, ZO-1 and β-catenin; (B3) F-actin, ZO-1 and αN-catenin. Upper rows, low magnification images; lower rows, high magnification images of the boxed areas in the upper rows. Bars, upper rows 10 µm; lower rows 2.5 µm. Arrowheads indicate dendritic filopodia-filopodia contact sites. (C) Quantitation of the co-localization of the components of the nectin and cadherin systems with ZO-1 in control neurons. In each experiment, 30 punctate immunofluorescence signals for F-actin at dendritic filopodia-filopodia contact sites were randomly chosen and the percentage of the punctate signal for ZO-1, nectin-1, nectin-3, afadin, N-cadherin, β-catenin or αN-catenin, which was co-localized with that for F-actin (% of co-localization), was counted.