Regulation of Dendritic Filopodial Interactions by ZO-1 and Implications for Dendrite Morphogenesis
Figure 2
Localization of ZO-1 at the tips of free dendritic filopodia and filopodia-filopodia contact sites in cultured hippocampal neurons.
Triple immunostaining for F-actin, ZO-1 and either Tau-1 or MAP2 in cultured hippocampal neurons. Cultured neurons on 5 DIV were triple-stained for F-actin, ZO-1 and either Tau-1 or MAP2. (A) F-actin, ZO-1 and Tau-1; (B) F-actin, ZO-1 and MAP2. Upper rows, low magnification images; lower rows, high magnification images of the boxed areas in the upper rows. Bars, upper rows 10 µm; lower rows 2.5 µm. Dendrites and axons were identified by the signals for MAP2 and Tau-1. Arrows indicate the growth cones of dendrites. Double arrowheads and arrowheads indicate the tips of free dendritic filopodia and dendritic filopodia-filopodia contact sites, respectively. It is noted that multiple Tau-1-positive neurites were observed in the neurons shown in (A) as described previously [34]. One of these multiple Tau-1-positive neurites is specified as an axon in the later stages of neuronal development [34].