Ethanol Metabolism Modifies Hepatic Protein Acylation in Mice
Figure 2
Mitochondrial extracts were isolated from livers of wild-type or SIRT3KO mice fed a standard or ethanol diet for 6-8 weeks and analyzed for total protein acetylation (A), propionylation (B), butyrylation (C), and succinylation (D) by western blot analysis with an acyllysine-specific antibody; normalized to total mitochondrial content using anti-voltage-dependent anion channel (VDAC); n = 4 mice/condition.