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Performance Comparison of Digital microRNA Profiling Technologies Applied on Human Breast Cancer Cell Lines

Figure 1

Project design.

Relevant figure and table references are noted. Human breast cell lines were cultured and total RNA was extracted. MiRNA profiles were obtained using four different platforms; Exiqon miRCURY LNA, Life Technologies SOLiD4, Illumina HiSeq, and NanoString nCounter. A local miRNA database (Exma-miRDB) was generated based on mature sequences found in miRBase v17. The performances of the platforms were evaluated in regards to accuracy, sensitivity, and flexibility.

Figure 1

doi: https://doi.org/10.1371/journal.pone.0075813.g001