The Formin Protein mDia2 Serves as a Marker of Spindle Pole Dynamics in Vitrified-Warmed Mouse Oocytes
Figure 5
Localization of mDia2 and tubulin at the time of thawing.
Vitrified MII oocytes were stored in LN2 for 2 weeks. Oocytes were taken out from LN2, incubated in decreasing concentrations of sucrose, and then fixed immediately (0 h). Some oocytes were incubated in 37 C for recovery for 3 h after thawing (3 h). These oocytes were subjected to immunofluorescence staining with anti-mDia2 and anti-α-tubulin antibodies. Green, mDia2; red, α-tubulin. In the oocyte at 0 h, mDia2 localization at spindle poles is not visible because the image is focused to MTOCs with emanating microtubules. Goat IgG was used instead of anti-mDia2 antibody in double immunofluorescence staining as a negative control (bottom right).