Comparison of Two Voltage-Sensitive Dyes and Their Suitability for Long-Term Imaging of Neuronal Activity
Figure 8
RH795 had weaker pharmacological and phototoxic effects than Di-4-ANEPPS.
Separate ganglia were used for each dye and concentration. During the experiment the pyloric rhythm was recorded extracellularly from the dvn and the instantaneous firing frequencies of LP were monitored. Frequencies were measured for 60 seconds each, every 10 minutes for one hour, than at 90 minutes, 120 minutes and 180 minutes. Colors code differences from a control measurement before dye application. Cool colors indicate small deviations from control and hot colors represent large deviations. Data from one animal is represented in one line, from left to right. Preparations that experienced the same treatment are highlighted with the same grey boxes (N = 3). The saline control is always represented by the bottom three lines highlighted in white. Firing frequency classes are shown by columns (left to right: 0–2 Hz, 3–9 Hz and 10–55 Hz).