Comparison of Two Voltage-Sensitive Dyes and Their Suitability for Long-Term Imaging of Neuronal Activity
Figure 7
Dye toxicity and phototoxicity increased over time.
A. Example of an extracellular recording of the triphasic pyloric rhythm on the dvn showing the activity patterns of PD, LP and PY and plot of instantaneous firing frequency (f.f.) of LP. Top: saline control. Bottom: after 180 minutes exposed to Di-4-ANEPPS (0.1 mM). Arrows indicate extra LP spikes. B. The distribution of LP instantaneous spike frequencies changed over time. The frequencies ranges given are 0–2 Hz, 3–9 Hz and 10–55 Hz. The firing frequency distribution changed for RH795, between different conditions (saline control and 0.05 mM, 0.1 mM and 0.2 mM RH795) and for illuminated and non-illuminated preparations (N = 3, measurements every ten minutes for one hour, then at 90 minutes, 120 minutes and 180 min). Each bar includes data from three experiments. C. Similar to B., for Di-4-ANEPPS.