Comparison of Two Voltage-Sensitive Dyes and Their Suitability for Long-Term Imaging of Neuronal Activity
Figure 5
Di-4-ANEPPS and RH795 showed bleaching over time.
A. Photos of STGs taken every 10 minutes during 60 minutes of constant illumination. Ganglia had been stained for 24 hours with 0.1(top) or 0.05 mM Di-4-ANEPPS (bottom) prior to light exposure. Camera shutter speed was 200 msec for RH795 and 100 msec for Di-4-ANEPPS. B. Bleaching was time-dependent for both dyes (Two-way ANOVA: p<0.0001; N = 12). Di-4-ANEPPS bleached faster than RH795 (Two-way ANOVA: p<0.05; N = 12). Mean grey values were normalized to the maximum and plotted over the duration of illumination. C. Comparison of the normalized mean grey values after 60 min of illumination, for all concentrations used (green: RH795; orange: Di-4-ANEPPS).