Comparison of Two Voltage-Sensitive Dyes and Their Suitability for Long-Term Imaging of Neuronal Activity
Figure 4
The S/N of RH795 is independent of LED strength.
A. Left: S/N for RH795 did not change with excitation light strength (n = 12 trials in N = 3 animals, p>0.05). Right: S/N for Di-4-ANEPPS depended on excitation light strength (n = 10 trials in N = 3 animals, p<0.05). B. Fluorescence emission (mean grey values) increased with increasing excitation light strength for Di-4-ANEPPS (left; p<0.05; n = 22 trials from N = 4 animals), but not for RH795 (right; p>0.05; n = 23 trials from N = 4 animals).