Dissection of Malonyl-Coenzyme A Reductase of Chloroflexus aurantiacus Results in Enzyme Activity Improvement
Figure 5
Site-directed mutagenic analysis of the MCR-N and MCR-C proteins.
(A) The location of amino acid changed in the MCR-N and MCR-C proteins that were tested. (B) In vitro analysis of malonyl-CoA reductase activity. Wild-type and mutated proteins were tested and the products were identified by LC-MS. In MCR-N reactions, wild-type MCR-C protein was used to convert malonyl-CoA into malonate semialdehyde, which is the substrate of MCR-N.