Cadherin 6 Is a New RUNX2 Target in TGF-β Signalling Pathway
Figure 5
TGF-β dependent CDH6 induction is mediated by RUNX2.
A) qRT-PCR analysis of CDH6 levels in non-treated (NT) and TGF-β treated (TGF) Nthy.ori cells, in presence of DMSO (mock, white bars), Actinomycin D (grey bars), cycloheximide (black bars). The bars represent the fold expression of the CDH6 mRNA in the indicated samples normalized to the GAPDH levels. The results are the average of two different replicates. B) qRT-PCR analysis of CDH6 levels in Nthy.ori 3.1 cells non-treated (black bars) or treated with TGF-β (white bars) after transfection with RUNX2 siRNA (right) or control siRNA (left). For both control siRNA and RUNX2 siRNA-treated samples, the bars represent the relative fold change of CDH6 upon TGF-β treatment as compared to non-treated cells. The graphs show one representative experiment. The experiment was replicated three times, obtaining comparable results. C and D) qRT-PCR analysis of RUNX2 and CDH6 mRNA levels in B-CPAP (C) and TPC1 (D) cells non-treated (NT, black bars) or treated with 10mM SB-431542 for 24h (grey bars) or 48h (white bars). Expression levels of CDH6 and RUNX2 were normalized to the geometric mean of GAPDH and CYPA. p-value was calculated by two-tailed Student’s t-test. *** p≤ 0.001; ** p≤ 0.01; * p≤ 0.05.