Dietary Oleic Acid Increases M2 Macrophages in the Mesenteric Adipose Tissue
Figure 6
HMF diet induces peritoneal cell adhesion.
(A) F4/80 and CD206 expression, by flow cytometry, on peritoneal macrophages, and SVF macrophages isolated from unwashed or washed MAT. F4/80Int CD206+ (a) and F4/80HI CD206− (b) macrophage populations are boxed. (B) Total cell counts (per mouse) of peritoneal cells (PCs) or macrophages (Macs) isolated by peritoneal lavage following three day control or milk-fat feeding. (N = 3) (C) C57BL6, MCP-1−/−, or ICAM-1−/− mice were fed control or HMF diet. F4/80 gene expression from HMF unwashed MAT is expressed as fold change relative to mice on control diet. (N = 5–8) (D) F4/80 and MCP-1 gene expression (qPCR, relative to Gapdh) in the washed MAT of mice fed a three day control or HMF diet. (N = 10,11).