Biosynthesis of Sandalwood Oil: Santalum album CYP76F Cytochromes P450 Produce Santalols and Bergamotol
Figure 5
GCMS analysis of products formed in vitro with clade II SaCYP76Fs.
GCMS analysis (extracted ion chromatograms) of products formed in vitro with (A) SaCYP76F38v1; (B) SaCYP76F38v2; (C) SaCYP76F37v1; (D) SaCYP76F37v2. Assays were performed with a sesquiterpene mixture of α-, β- and epi-β-santalene and α-exo-bergamotene (Figure S3) as substrate and microsomes prepared from yeast transformed with SaCPR, individual clade II candidate SaCYP76F cDNAs, or (E) empty vector as control. Peak numbers match the numbers in Table 1 and Figure 1.