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Biosynthesis of Sandalwood Oil: Santalum album CYP76F Cytochromes P450 Produce Santalols and Bergamotol

Figure 4

GCMS analysis of products formed in vitro with clade I SaCYP76Fs.

GCMS analysis (extracted ion chromatograms) of products formed in vitro with (A) SaCYP76F39v2; (B) SaCYP76F40; (C) SaCYP76F41; (D) SaCYP76F42. Assays were performed with a sesquiterpene mixture of α-, β- and epi-β-santalene and α-exo-bergamotene (Figure S3) as substrate and microsomes prepared from yeast transformed with SaCPR, individual clade I candidate SaCYP76F cDNAs, or (E) empty vector as control. Peak numbers match the numbers in Table 1 and Figure 1.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0075053.g004