Heterogeneity of the MDCK Cell Line and Its Applicability for Influenza Virus Research
Figure 5
Plaque size of virus variants with α2–3 or α2–6 binding preferences in MDCK clones.
Variants of influenza B virus strain B/Victoria/504/2000 with binding preferences for sialylated glycans with either α2–3 (orange bars) or α2–6 (green bars) glycosidic linkage were used as probes to investigate the cell clones for their support of replication of viruses with different receptor-binding properties. Bars represent the diameter (mm) of virus plaques in the corresponding cell clones 96 hours post infection, at 33°C. Values represent the Mean ± SE from two independent experiments. Asterisk (*) indicates a significant difference (P<0.01) in comparison with the corresponding values in the parental MDCK cells.