Kinetics of Germination of Individual Spores of Geobacillus stearothermophilus as Measured by Raman Spectroscopy and Differential Interference Contrast Microscopy
Figure 5
Germination of multiple individual G. stearothermophilus spores with AGFK at different temperatures.
Heat activated (30 min, 100°C) spores were germinated at various temperatures with AGFK and 10 mM sodium phosphate buffer (pH 8.0), and germination of individual spores was monitored by DIC microscopy as described in Methods. Germination of ≥220 individual spores at 65°C (▴), 55°C (•), or 45°C (▪) was shown in (a). Kinetics of germination of ten individual spores at 65°C (b), 55°C (c), or 45°C (d) was given in (b–d).