Sodium Butyrate Induces Growth Inhibition and Apoptosis in Human Prostate Cancer DU145 Cells by Up-Regulation of the Expression of Annexin A1
Figure 4
The effect of NaB treatment on siANXA1-transfected cells.
(A) Western blot analysis of ANXA1 expression in siANXA1 and siMock cells after NaB treatment. Data were normalized with β-actin values and are expressed as fold changes of β-actin in NaB treated group relative to control. (B) The proliferation of siANXA1 and siMock cells after NaB treatment. (C) Western blot analysis of the expression of Bax and Bcl-2 in siANXA1 and siMock cells. Bcl-2 and Bax expression levels were normalized to β-actin and are presented as the Bax/Bcl-2 ratio. All experiments were repeated at least three times. Data are means ± SEM. (n = 3) (Dunnett’s test, *P<0.05 vs. control).