Aluminum Hydroxide Adjuvant Differentially Activates the Three Complement Pathways with Major Involvement of the Alternative Pathway
Figure 5
Time course of anaphylatoxin generation by Al(OH)3 in serum.
The anaphylatoxins were measured in NHS after incubation with 30(OH)3 at 37°C for the indicated times and removal of the Al(OH)3-containing precipitate by centrifugation. High and low positive controls provided by the manufacturer and NHS without addition of Al(OH)3 were included as controls in all experiments. The figure shows means±SD of 2 independent experiments each with double determinations. P-values for the production of anaphylatoxins after 60 minutes were calculated using 2-way ANOVA and Bonferroni posttest (*p<0.05; ***p<0.001).