Effects of Elaidic Acid on Lipid Metabolism in HepG2 Cells, Investigated by an Integrated Approach of Lipidomics, Transcriptomics and Proteomics
Figure 2
The FA composition of HepG2-SF phospholipids analyzed by gas chromatography.
The composition of phospholipids from HepG2-SF cells incubated 6 days in 100 µM oleic, elaidic, or stearic acid supplemented medium were determined. Panel A and Panel B are the high and low abundant FAs present in the PLs, respectively. Each bar represents the average of three biological replicas. The y-axis is the percent of total FA methyl esters measured. The x-axis indicates the different PL FAs measured as their methyl esters. Color coding corresponds to the different supplemented FFAs. Panel C shows a heatmap representation of the distribution of FAs in PLs of the HepG2-SF cells after supplementation. The FA composition of HepG2-SF phospholipids change depending on the different supplemented fatty acids, Stearic and Oleic profiles are alike whereas the Elaidic FA PL profile differs from all the other groups. * marks measurements significantly differing from controls using the unpaired t-test, two-tailed with a 95% confidence interval.