Luciferase Reporter Gene Assay on Human, Murine and Rat Histamine H4 Receptor Orthologs: Correlations and Discrepancies between Distal and Proximal Readouts
Figure 7
Inhibition of the response to histamine and clozapine by JNJ7777120.
Concentration response curves of histamine (A) and clozapine (B) alone and in the presence of JNJ7777120 at increasing concentrations, determined on hH4R expressing HEK293T-CRE-Luc cells in the luciferase reporter gene assay, and corresponding Schild plots (C). The pA2 values determined for JNJ 7777120 from Schild regression were 8.39 (slope: 0.83±0.02) and 8.17 (slope: 0.45±0.01) versus histamine and clozapine, respectively. Data points shown are the mean ± SEM of at least three (histamine) or five (clozapine) independent experiments performed in triplicate.