Luciferase Reporter Gene Assay on Human, Murine and Rat Histamine H4 Receptor Orthologs: Correlations and Discrepancies between Distal and Proximal Readouts
Figure 4
Effect of histamine and thioperamide on the luciferase activity in hH4R expressing cells.
Concentration-response curves of histamine (HA) and thioperamide (THIO) on HEK293T-SF-hH4R-His6-CRE-Luc cells, stably co-expressing the CRE-controlled luciferase and the hH4R. The cells were pre-stimulated with 500 nM of forskolin alone or in combination with IBMX (50 µM). The effect of forskolin or that of forskolin plus IBMX was defined as 100% luciferase activity. Data points shown are the mean ± SEM of two independent experiments performed in triplicate.