Luciferase Reporter Gene Assay on Human, Murine and Rat Histamine H4 Receptor Orthologs: Correlations and Discrepancies between Distal and Proximal Readouts
Figure 3
Inhibition of luciferase activity by histamine in rH4R expressing cells.
Gαi/o mediated inhibition of forskolin (0.5 µM–5.0 µM) stimulated luciferase activities by histamine (HA) in HEK293T-SF-rH4R-His6-CRE-Luc cells, stably expressing the rH4R and the CRE-controlled luciferase. (A) Representative luciferase reporter gene with RLU values as readout. (B) Normalized inhibition of forskolin stimulated luciferase activity (100%) by histamine (HA), with the maximum inhibitory effect of which set at 0%. Data points shown are the mean ± SEM of at least three independent experiments performed in triplicate.