Molecular Characterization of UGT94F2 and UGT86C4, Two Glycosyltransferases from Picrorhiza kurrooa: Comparative Structural Insight and Evaluation of Substrate Recognition
Figure 2
Phylogenetic tree of deduced amino acid sequences of UGT86C4 and UGT94F2.
Phylogeny of UGTs was inferred using neighbour-joining method of the MEGA 5.1 software. A total of 20 protein sequences used for analysis were from following plant species: Picrorhiza kurrooa UGT94F2 (JQ996409), Picrorhiza kurrooa UGT86C4 (JQ996408), Arabidopsis thaliana UDP-glycosyltransferase 86A1 (AAM91353), Linum usitatissimum UDP-glycosyltransferase 1 (AFJ53011), Dianthus caryophyllus UDP-glucosyltransferase (BAF75888), Lycium barbarum UDP-glucose:glucosyltransferase (BAG80548), Gardenia jasminoides UDP-glucose glucosyltransferase (BAK55737) Catharanthus roseus UDP-glucose iridoid glucosyltransferase (BAK55749), Medicago truncatula cytokinin-O-glucosyltransferase (XP_003615832), Veronica persica flavonoid glycosyltransferase UGT94F1 (BAI44133), Gardenia jasminoides UDP-glucose glucosyltransferase (BAM28984), Catharanthus roseus UDP-glucose:flavonoid glucoside 1,6-glucosyltransferase (BAH80312), Panax notoginseng UDP-glycosyltransferase (AED99884), Sesamum indicum UDP-glucose:sesaminol 2′-O-glucoside-O-glucosyltransferase (BAF99027), Bupleurum chinense glycosyltransferase UGT4 (AFK79036), Zea mays anthocyanidin 3-O-glucosyltransferase (ACG26485), Ginkgo biloba UDP-glucose:flavonoid glucosyltransferase (AEQ33588), Triticum urartu flavanone 7-O-glucoside 2′′-O-beta-L-rhamnosyltransferas (EMS55799), Medicago truncatula anthocyanidin 3-O-glucosyltransferase (AES75297), Vitis vinifera UDP-glycosyltransferase 86A1(XP_002276858).