Geometry-Driven Cell Organization Determines Tissue Growths in Scaffold Pores: Consequences for Fibronectin Organization
Figure 2
Filling of scaffold pores by fibroblasts for three pore size categories and three time points.
Confocal microscopy for visualization of actin cytoskeleton (green) and cell nuclei (blue) to illustrate the filling of scaffold pores by human fibroblasts at day 1, 3, and 7 for the three pore size categories (scale bar equals 100 µm). Data shown corresponds to the maximum projection images of the 3D image stacks recorded inside scaffolds. During the imaging, scaffold pores could be identified due to background staining of the scaffold material and are highlighted by dashed lines.