Novel Small-Molecule AMP-Activated Protein Kinase Allosteric Activator with Beneficial Effects in db/db Mice
Figure 5
The effects of ZLN024 in rat primary hepatocytes.
(A) Concentration response of the effects on AMPK and ACC phosphorylation due to ZLN024 treatment for 3 hr in rat primary hepatocytes. (B) ZLN024 did not change the ADP/ATP ratio after incubation for 3 hr in rat primary hepatocytes, CCCP (1µmol/l) was used as a positive control (n = 3). (C) Inhibition of insulin-stimulated (10 nmol/l) fatty acid synthesis by 24 hr of exposure to ZLN024. (D) The effects of treatment with ZLN024 for 21 hr on the gene expression of FAS (n = 3). (E) AMPK α1/α2-DN adenovirus expression reversed ACC phosphorylation and fatty acid synthesis (n = 2) (F) caused by ZLN024 (20 µmol/l) effectively for 24 hr treatment (n = 2). *, P<0.05, **, P<0.01 compared with the untreated control.