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Novel Small-Molecule AMP-Activated Protein Kinase Allosteric Activator with Beneficial Effects in db/db Mice

Figure 4

ZLN024 stimulates glucose uptake and fatty acid oxidation in L6 myotubes.

(A) ZLN024 increases glucose uptake in L6 myotubes after incubation for 3 hr. Insulin (100 nmol/l) was added for the final 30 min of the experiment (n = 4). (B) ZLN024 increases fatty acid oxidation in L6 myotubes after incubation for 4 hr. AICAR (1 mmol/l) was used as a positive control (n = 3). (C) Effects of AMPK α1/α2-DN adenovirus expression on ACC phosphorylation and glucose uptake (D) caused by ZLN024 (20 µmol/l) (n = 4). #, P<0.1, *, P<0.05, **, P<0.01 compared with the untreated control.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0072092.g004