A Novel Golgi Retention Signal RPWS for Tumor Suppressor UBIAD1
Figure 5
RPWS as the Golgi retention signal of UBIAD1.
A, A series of deletion clones were constructed for UBIAD1-EGFP (i.e., 20AA, Δ20, the first 20 amino acids of UBIAD1 N terminus was deleted); B, Multiple alignment of the N terminus of the UBIAD1 putative orthologs from ten different species (including Drosophila melanogaster, Anopheles gambiae str. PEST, Danio rerio, Gallus gallus, Canis lupus familiaris, Bos Taurus, Homo sapiens, Pan troglodytes, Mus musculus, Rattus norvegicus) using program Geneious. RPWS is conserved across all ten species; C, RPWS (amino acids 54 to 57) of the UBIAD1 N terminus was predicted to be the Golgi retention signal by Signal IP program. Arrows indicate the predicted Golgi retention signal. D, Deletion clones made in A were sequentially transfected into the HEK293 cells. Arrows point to the Golgi. WT, wild type UBIAD1-EGFP; Δ20, the first 20 amino acids of UBIAD1 N terminus was deleted.