M1 Muscarinic Receptor Activation Mediates Cell Death in M1-HEK293 Cells
Figure 9
Schematic diagram showing that M1-induced cell death involved two components.
The major component of death was ERK-independent and occurred within hours of carbachol activation of the cells. This pathway was not blocked by the MEK inhibitor UO126 and occurred prior to activation of cleaved caspase-3. In contrast the minor component, which was slower and more progressive, was blocked by UO126 and associated with cleaved caspase-3 expression. Both pathways were fully blocked by the M1 antagonist and only occurred in HEK-M1 cells stably expressing M1 receptors.