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M1 Muscarinic Receptor Activation Mediates Cell Death in M1-HEK293 Cells

Figure 8

Western blots of pERK (A) and EGR-1 (B) at 10 min, 1 hr and 24 hours after Carbachol +/− U0126.

A, pERK Western showing ERK phosphorylation at 10-min and 24 hours after addition of carbachol to HEK293-M1 cells. The MEK blocker U0126 completely inhibited this ERK phosphorylation. B, EGR-1 induction at 1 hour and 24 hours after addition of carbachol to HEK293-M1 cells. The MEK blocker U0126 completely inhibited this EGR-1 induction.

Figure 8

doi: https://doi.org/10.1371/journal.pone.0072011.g008