M1 Muscarinic Receptor Activation Mediates Cell Death in M1-HEK293 Cells
Figure 7
Activation of pERK and EGR-1 is still evident 24 hours following initial carbachol treatment.
HEK293-M1 cells were stimulated for 24 h with vehicle or 100 µM carbachol as indicated. Then the cells were fixed and stained for pERK and EGR1. Note the high uniform activation of pERK and EGR-1. The lower panels show the effect of pretreatment (30 minutes) with 5 µM UO126, which shows that the aberrant signalling present at 24 h is independent of the MEK signalling pathway. Graphs show quantification of the induction and reversal of pERK and EGR-1 with U0126.