M1 Muscarinic Receptor Activation Mediates Cell Death in M1-HEK293 Cells
Figure 5
Analysis of carbachol mediated cell death using xCELLigence technology.
HEK293-M1 cells were stimulated with various concentrations of carbachol (A), in the presence (or absence) of a M1 selective antagonist (B), and with 5 µM UO126 (C and D). Panel D shows the period immediately following drug addition to highlight the immediacy of the carbachol response. In each graph the black arrow shows the time point the respective drugs were added. Cellular measurements are made continuously in real time, where the level of total cell adhesion is represented as Cell Index. Higher Cell Index levels represent more adhesion, whereas a reduction in Cell Index represents a loss of adhesion. In all four graphs the red and blue Cell Index curves are the same and represent vehicle-treated and 100 µM carbachol-treated HEK293-M1 cells. The reduction in adhesion mediated by carbachol is immediate, concentration-dependent, blocked by M1 antagonist, but not blocked by UO126 (MEK blocker). xCELLigence data show representative Cell Index curves of at least six independent experiments.