M1 Muscarinic Receptor Activation Mediates Cell Death in M1-HEK293 Cells
Figure 3
Acute activation of EGR-1 by M1 receptors is ERK dependent in HEK293-M1 cells.
HEK293-M1 cells were pretreated with 5 µM UO126 (or vehicle) for 30 mins prior to the addition of 100 µM carbachol for the activation of pERK (10 mins) and EGR-1 (1 h). Data are representative of at least three independent experiments. Scale bar: 50 µM. Graphs show quantification of the induction and reversal of pERK and EGR-1 with U0126.