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M1 Muscarinic Receptor Activation Mediates Cell Death in M1-HEK293 Cells

Figure 2

pERK1/2 and EGR-1 induction in transfected HEK293-M1 cells by carbachol.

Expression of pERK1/2 and EGR-1 after short term carbachol treatment in HEK293-Vec cells and HEK293-M1 cells. Cells were stimulated for 10 minutes and 1 h for pERK and 1 h for EGR-1 expression with 100 µM carbachol or vehicle. Data are representative of at least three independent experiments. Scale bar: 50 µm.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0072011.g002