Determination of Neuraminidase Kinetic Constants Using Whole Influenza Virus Preparations and Correction for Spectroscopic Interference by a Fluorogenic Substrate
Figure 5
NA enzyme kinetics curves with and without spectroscopic corrections.
(A) Data for A/PR/8/34 (H1N1) influenza virus; (B) A/CA/04/09 (H1N1pdm09) influenza virus and (C) B/WIS/01/10 influenza virus. Each data point represents the mean of 4 (A/CA/04/09 and B/WIS/01/10 viruses) or 7 (A/PR/8/34 virus) independent measurements. Dotted lines represent data without spectroscopic correction and solid lines represent data fitting to a hyperbolic equation after correction.