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S-Nitrosoglutathione Reductase Inhibition Regulates Allergen-Induced Lung Inflammation and Airway Hyperreactivity

Figure 6

Treatment with the GSNOR inhibitor reduced CCL11 production during allergic lung inflammation.

LMCs were obtained from control mice and Th2 recipient mice that have been treated with either SPL-334 or vehicle and inhaled OVA for 7 d. (A) LMCs were stimulated with anti-CD3 (2 µg/ml) or OVA323–334 peptide (1 µg/ml) for 24 h and supernatant analyzed for eotaxin production by ELISA. (B) CCL11 levels in the BALF were measured by ELISA. Results are mean ±SE of four to six individual mice analyzed per group in triplicates and are representative two independent experiments. *p, 0.05, compared with Th2 group.

Figure 6

doi: https://doi.org/10.1371/journal.pone.0070351.g006