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S-Nitrosoglutathione Reductase Inhibition Regulates Allergen-Induced Lung Inflammation and Airway Hyperreactivity

Figure 5

Treatment with the GSNOR inhibitor reduced Th2 cytokine production during allergic airway inflammation.

LMCs were obtained from control mice and Th2 recipient mice that have been treated with either SPL-334 or vehicle and inhaled OVA for 7 d. LMCs were stimulated with anti-CD3 (2 µg/ml) or OVA323–334 peptide (1 µg/ml) for 24 h and supernatant analyzed for IL-4, IL-5 or IL-13 production by ELISA. Results are mean ±SE of four mice analyzed per group in triplicates and are representative two independent experiments. *p, 0.05, compared with Th2 group.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0070351.g005