Cep63 and Cep152 Cooperate to Ensure Centriole Duplication
Figure 6
Lack of centrosomal Cep63–Cep152 causes a delay in HsSAS-6 recruitment.
(A) Cep63 is present at the PCM before HsSAS-6 recruitment. Telophase, G1 phase, S or G2 phase and mitotic HeLa cells, as indicated, were stained with anti-centrin 2 (red), Cep63 (green), and HsSAS-6 (blue) antibodies. Centrosomes from each cell are shown. Scale bar 1 µm. (B) HsSAS-6 foci were counted in U2OS cells with nuclear PCNA foci (a marker of DNA replication) after 96 hours RNAi as indicated, n >150, 3 experiments. P values from a students’ t-test are indicated on the graph. (C) U2OS cells were stained with anti-Cyclin A and HsSAS-6 antibodies after control, Cep63, or Cep152 RNAi. Cyclin A status, negative (-, early G1 phase), dull (+, G1-S), or bright (++, S-G2), and the number of HsSAS-6 foci (0,1,2,>2) were scored in asynchronous populations in three independent experiments, n >150.