Cep63 and Cep152 Cooperate to Ensure Centriole Duplication
Figure 5
Cep63-Cep152 centrosomal recruitment is downstream of Cep192.
(A-D) Control RNAi or RNAi of Cep63, Cep152, or Cep192, was carried out for 4 days in U2OS cells, followed by immunofluorescence on replicate samples, with anti- γ-tubulin and anti-Cep63 (A), Cep152 (B), or Cep192 (C) antibodies. Fluorescence intensity of Cep63, Cep152, Cep192, and γ-tubulin at the centrosome were measured (graphs A-D). All intensity measurements were normalised to the mean of the control population and p values are indicated above (students’ t-test). (E-G) Images of γ-tubulin and Cep63, Cep152, or Cep192 immunofluorescence at the centrosome from the experiment shown in A-D. Scale bar 1 µm. (H) Western blots of whole cell lysates from U2OS cells used in experiments (A-G) using anti-Cep152 (Bethyl) and α-tubulin antibodies.