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Assessing Gibberellins Oxidase Activity by Anion Exchange/Hydrophobic Polymer Monolithic Capillary Liquid Chromatography-Mass Spectrometry

Figure 4

Extracted ion chromatogram of five GA standards.

Shown in inset is the total ion chromatogram of five GA standards. Experimental conditions: column, poly(META-co-DVB-co-EDMA) monolithic column (30-cm long, 100 µm i.d., 360 µm o.d.); flow rate, 800 nL/min; mobile phase, ACN/H₂O/FA (60/40/0.6, v/v/v). Order of peaks: 1. GA₁, 2. GA₂₀, 3. GA₅₃, 4. GA₄, 5. GA₉.

doi: https://doi.org/10.1371/journal.pone.0069629.g004