Norepinephrine Inhibits Macrophage Migration by Decreasing CCR2 Expression
Figure 5
NE regulates antigen uptake by BMM.
The whole bone marrow cells from C57BL/C mice were cultured with M-CSF in the presence of NE (1 x 10-8 M and 1 x 10-6 M) for 7 days. At day 7, FITC-Dextran (200µg/mL) was added to the culture for further 30 min culture at 37°C. As a negative control, FITC-Dextran-added wells were incubated at 4°C for 30 min. At end of the experiment, cells were collected and stained with antibodies for CD11b and F4/80. The percentage of FITC-Dextran-positive CD11b+/F4/80+ BMM was obtained by gating on CD11b+/F4/80+ BMM. Representative flow cytometric data are shown in (A) and corresponding graphic data are shown in (B). Bars represent FITC-Dextran-positive DCs as the mean of 4 independent experiments ± SD. *p<0.05; ** p<0.01, compared to untreated control (0 M).