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Norepinephrine Inhibits Macrophage Migration by Decreasing CCR2 Expression

Figure 4

LPS exacerbates NE’s effects.

In the last 24 hours of 7 day culture of BM cells with the treatment of NE, LPS (50 ng/mL) was added to the culture. At the end of culture, cells were collected and stained with Abs for CD11b, MHC II and CCR2 antibodies. Representative dot plot data and their corresponding graphic format data of percentage of MHC II+/F4/80+ Mφ are shown in (A) and (B) respectively. Representative dot plot data and their correspondent graphic format data of percentage of CCR2+/F4/80+ Mφ shown in (C) and (D) respectively. Data show mean ± SD of 4 independent experiments. Significant difference is indicated as * p<0.05 and ** p<0.01, compared to untreated control.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0069167.g004