Norepinephrine Inhibits Macrophage Migration by Decreasing CCR2 Expression
Figure 3
NE inhibits macrophage migration by decreasing CCR2 expression.
BM cells were cultured in hormone-deficient medium with murine M-CSF alone, or in the presence of varying concentrations of NE. At day 7, cells were collected and stained with Abs for CD11b, F4/80 and CCR2. Based on the gating of CD11b+/F4/80+ cells, representative dot plot for percentage of CCR2+/F4/80+ Mφ is shown in (A). The corresponding graphic format data of percentage of CCR2+/F4/80+ Mφ is shown in (B). The comparison of cells that passed through insert filter after 4 hours incubation with MCP-1 (final concentration, 50 ng/mL) in the lower chamber are shown in Figure 2 (C). Data show mean ± SD of 4 independent experiments. Significant difference is indicated as * p<0.05, compared to untreated control.