Norepinephrine Inhibits Macrophage Migration by Decreasing CCR2 Expression
Figure 2
High dose of NE decreases CSF-1R expression.
Unfractionated BM cells were cultured with murine M-CSF alone, or in the presence of varying concentrations of epinephrine. At day 7, cells were collected and stained with Abs for CD11b, F4/80 and CSF-1R. Based on the gating of CD11b+/F4/80+ BMM cells, representative histogram data of CSF-1R expression on Mφ is shown in (A). The corresponding graphic format data of CSF-1R expression on Mφ is shown in (B). Their corresponding MFI of CSF-1R expression on Mφ is shown in (C). Data show mean ± SD of 4 independent experiments. Significant difference is indicated as * p<0.05, compared to untreated control.