Norepinephrine Inhibits Macrophage Migration by Decreasing CCR2 Expression
Figure 1
NE regulates macrophage proliferation and maturation from BM.
Unfractionated BM cells were plated in a 24-well plate at 2 x 106 cells/well and cultured for 7 days in hormone-deficient medium with murine M-CSF alone, or in varying concentrations of NE (1 x 10-8 M or 1 x 10-6 M) added at day 0, or in the presence of 1 x 10-6 M of NE added at different time points (day 0, 3 and 6). BMM proliferation was examined using the CyQUANT Cell Proliferation Assay Kit. Briefly, 2 x 105 BM cells were seeded into each well of a 96-well plate and treated with various doses of NE or without NE. At day 7, cells were collected and total cell numbers were counted based on the standard curve (A). To phenotype BMM, cells stained with Abs for CD11b, MHC II and F4/80. Representative dot plot data of the percentage of MHC II+/F4/80+ Mφ are shown in (B). The graphic format of the percentage of MHC II+/F4/80+ Mφ is shown in (C). The time course of NE effects is shown in (D). Data show mean ± SD of 4 independent experiments. Significant difference is indicated as * p<0.05 or **p<0.01, compared to untreated control or different time points.