Cell Type-Specific Dependency on the PI3K/Akt Signaling Pathway for the Endogenous Epo and VEGF Induction by Baicalein in Neurons versus Astrocytes
Figure 2
Effects of 12/15-LOX knockdown on the baicalein-induced Epo and VEGF gene expression.
(A) qRT-PCR of 12/15-LOX mRNA in neurons and astrocytes. (B) RT-PCR analysis of 12/15-LOX mRNA in neurons transfected with scrambled RNA (Scr) or siAlox15 for 72 h. (C, D) qRT-PCR of VEGF (C) and Epo (D) mRNA in siAlox15 or Scr-transfected neurons with or without 3.5 μM BA treatment for 12 h. In (A), ***p<0.01 versus Astrocytes group (n=3). In (C) and (D), *p<0.05 and **p<0.01 versus control (Ctrl); # p<0.05 versus the Scr-Ctrl (n=3).