LHX3 Interacts with Inhibitor of Histone Acetyltransferase Complex Subunits LANP and TAF-1β to Modulate Pituitary Gene Regulation
Figure 6
LANP represses LHX3-PIT1 synergism on target reporter genes.
(A) LANP influence on trans-activation of the Prl promoter by LHX3 and PIT1. A Prl enhancer/promoter luciferase reporter gene was transiently transfected into 293T cells with the indicated LHX3 and/or PIT1 expression vectors. Promoter activity was assayed by measurement of luciferase activity after 48 hours. Activities are mean [light units/10 seconds/µg total protein] of triplicate assays ± S.E.M. WT = wild type. a = significantly different to the LHX3+ PIT1+ pcDNA3.1 value (P<0.05). (B) Effects of LANP on the trans-activation of a luciferase reporter gene containing the mouse thyroid-stimulating hormone beta subunit (TSHβ/Tshb) gene promoter by LHX3 and PIT1. The indicated combinations of LHX3, PIT1, LANP or control expression vectors were transiently co-transfected into 293T cells. Promoter activity was assayed by measurement of luciferase activity after 48 hours. Activities are mean [light units/10 seconds/µg total protein] of triplicate assays ± S.E.M. a = significantly different to the LHX3+ PIT1+ pcDNA3.1 value (P<0.05).