LHX3 Interacts with Inhibitor of Histone Acetyltransferase Complex Subunits LANP and TAF-1β to Modulate Pituitary Gene Regulation
Figure 5
LANP represses LHX3 target promoters in pituitary GHFT1 cells.
(A) A Prl enhancer/promoter luciferase reporter gene was transiently co-transfected into GHFT1 cells with the indicated LHX3 and LANP expression vectors. a = significantly different to the LHX3+ pcDNA3.1 value (P<0.05). (B) The mouse thyroid-stimulating hormone beta subunit (TSHβ/Tshb) gene promoter reporter construct was transiently co-transfected into pituitary GHFT1 cells with the indicated LHX3 and LANP expression vectors. LHX3 and empty pcDNA3.1 vectors were co-transfected with the reporters to control for vector effects on LHX3 activity. Promoter activity was assayed by measurement of luciferase activity after 48 hours. Activities are mean [light units/10 seconds/µg total protein] of triplicate assays ± S.E.M. a = significantly different to the LHX3+ pcDNA3.1 value (P<0.05).