LHX3 Interacts with Inhibitor of Histone Acetyltransferase Complex Subunits LANP and TAF-1β to Modulate Pituitary Gene Regulation
Figure 4
Effects of INHAT proteins on LHX3a trans-activation of the mouse αGSU/Cga promoter.
(A, B) The indicated combinations LHX3, INHAT, CBP, or control expression vectors were transiently co-transfected into mouse pituitary GHFT1 cells with an αGSU (Cga) promoter luciferase reporter gene. Promoter activity was assayed by measurement of luciferase activity after 48 hours. Activities are mean [light units/10 seconds/µg total protein] of triplicate assays ± S.E.M. a = significantly different to the LHX3+ pcDNA3.1 value (P<0.05); b = significantly different to the LHX3+ CBP value (P<0.05).