LHX3 Interacts with Inhibitor of Histone Acetyltransferase Complex Subunits LANP and TAF-1β to Modulate Pituitary Gene Regulation
Figure 3
The LANP and TAF-1β INHAT proteins are associated with the LHX3-bound αGSU/Cga gene promoter.
Chromatin immunoprecipitation (ChIP) was used to probe occupancy of the pituitary glycoprotein binding element (PGBE) region of the mouse αGSU/Cga promoter [12], [48] by LANP, TAF-1β (A) or LHX3 (B) proteins in LβT2 pituitary cells. ChIP enrichment was measured by quantitative PCR and represented as percent input, calculated by 100*2∧(input - Ct (IP)). Values are mean ± SEM for three independent experiments. Immunoprecipitation with non-immune species-matched IgG were carried out as negative controls. a = significantly different to the IgG control value (P<0.05).